To investigate the anti-proliferative effects of Gamma-Tocotrienols (GTT) treatment on differential protein expression in HepG2 cells.
SHOW SIGNIFICANT ANTICANCER PROPERTIES IN COLON CANCER CELLS
STIMULATE CASPASE-3 AND CASPASE-9 THUS INDUCING APOPTOSIS IN CANCER CELLS
Several studies have been conducted on several isomers of Vitamin E, especially on the isoforms of Tocotrienols regarding their anticancer effects against hepatocellular carcinoma (HCC). The anti-proliferative effects of Tocotrienols, especially of the Delta and Gamma isoforms have been found to be quite significant.
Introduction to Liver Cancer and Delta-Tocotrienol
The liver is the largest internal organ in the body and is responsible for various functions in the body. IT has been estimated that in 2020 itself, in the United States of America, around 30,110 men and 12,649 women will be diagnosed with Liver cancer. The cases of liver cancer have been growing rapidly since 1980 as the cases have been tripled since that year. It has also been estimated that around 30,160 people in the USA, are going to die from liver cancer in 2020. Also, the death rate has been doubled from 1980 to 2017!
Since, the incidents of and the fatalities due to liver cancer are increasing rapidly, the need for a potent anticancer agent that can suppress cancer has risen. Several studies have been conducted on Tocotrienols, especially on Delta and Gamma isoforms to investigate their anticancer effects on Liver Cancer. In this study, the antiproliferative effects of Gamma-Tocotrienol on treatment on protein expression in HepG2 cells.
How does Gamma-Tocotrienol fight Leukaemia or Blood Cancer?
The study demonstrated that the Gamma-Tocotrienols treatment on HepG2 cells showed five differentially expressed proteins when compared to their respective untreated cells. Originally, for the untreated cells, two proteins were up-regulated, and three proteins were downregulated. One of the proteins which were upregulated was peroxiredoxin-4 (Prx4). Through GTT treatment, the Prx4 mRNA expression in HepG2 cells was decreased.
Hence, it was concluded that GTT might directly decrease the expression of peroxiredoxin-4, thereby suppressing the proliferation of liver cancer cells. Also, it has been observed that GTT also possesses lipid=lowering power in HCC which may also be the reason for its anticancer activities.
Reference for the Study in detail:
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Mohd Mutalip SS, et al. Biomolecules. 2019. PMID: 30634632
- Delta- and gamma-tocotrienol isomers are potent in inhibiting inflammation and endothelial activation in stimulated human endothelial cells.
Muid S, et al. Food Nutr Res. 2016. PMID: 27396399
- Norcantharidin combined with ABT-737 for hepatocellular carcinoma: Therapeutic effects and molecular mechanisms.
Ren J, et al. World J Gastroenterol. 2016. PMID: 27099439
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